rnjA [2017-08-17 14:43:33]

RNase J1

Locus: BSU14530

Isoelectric point: 5.90

Molecular weight: 61.34 kDa

Protein length: 555 aa

Gene length: 1665 bp

Function: RNA processing

Product: RNase J1

Essential: no

Synonyms: ykqC

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNases → Exoribonucleases
• Groups of genes → Essential genes

Gene

Coordinates: 1,523,118 → 1,524,785

Phenotypes of a mutant

• the mutant is strongly impaired in sporulation, genetic competence and many other phenotypes PubMed
• it is not possible to construct a rnjA rny double mutant PubMed

The protein

Catalyzed reaction/ biological activity

• endonuclease and 5'-3' exonuclease
• degrades SR5 and gapA RNAs PubMed
• degrades yflS mRNA PubMed

Protein family

• RNase J subfamily (according to Swiss-Prot)

Paralogous protein(s)

• RnjB

Effectors of protein activity

• initeraction with GapA-Sr1 stimulates activity PubMed

Structure

• 3ZQ4 PubMed

Localization

• cytoplasm, colocalizes with the ribosomes PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• required for thrS RNA processing, involved in maturation of the 5’-end of the 16S rRNA

Expression and Regulation

Operons

• Genes: rpoY-rnjA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• GP41 (rnjA under control of p(xyl)), available in Jörg Stülke's lab
• SSB342 (rnjA under pspac), cat, available in Harald Putzer lab
• GP2506 (rnjA::spc), available in Jörg Stülke's lab

Expression vector

• for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's lab
• for chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's lab
• pGP1441: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab

LacZ fusion

• pGP418 (in pAC7), available in Jörg Stülke's lab

GFP fusion

• GP1694 (in pHJS-105 PubMed), expression of rnjA-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
• GP1722 (in pBP43), expression of rnjA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed

FLAG-tag construct

• GP1020 (spc, based on pGP1331), available in Jörg Stülke's lab
• GP1075 (aphA3), available in Jörg Stülke's lab

Labs working on this gene/protein

• Harald Putzer, IBPC Paris, France Homepage
• David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage
• Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

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Original publications

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